Journal: Journal of Diabetes and Metabolic Disorders

Article Title: A case-control study to determination FBXW7 and Fetuin-A levels in patients with type 2 diabetes in Iraq

doi: 10.1007/s40200-021-00738-x

Figure Lengend Snippet: Clinical data in patients having diabetes mellitus (GI newly diagnosed DM, GII DM with metformin therapy) and control group

Article Snippet: While HbA1c was determined using Bio- Rad VARIANT Hemoglobin A1c programmer.

Techniques: Control, Significance Assay

Journal: Journal of Diabetes and Metabolic Disorders

Article Title: A case-control study to determination FBXW7 and Fetuin-A levels in patients with type 2 diabetes in Iraq

doi: 10.1007/s40200-021-00738-x

Figure Lengend Snippet: Correlation analysis of variables associated with serum FBXW7 protein level in the study populations

Article Snippet: While HbA1c was determined using Bio- Rad VARIANT Hemoglobin A1c programmer.

Techniques: Control

Journal: Advanced Science

Article Title: TRPC3 Regulates Islet Beta‐Cell Insulin Secretion

doi: 10.1002/advs.202204846

Figure Lengend Snippet: Metabolic Characterization of Trpc3 −/− Mice. a) Histological images of Langerhans islets from WT and Trpc3 −/− stained with H&E. Scale bars: 100 µm. b) Relative frequency of islet diameter in WT and Trpc3 −/− . c–e) Body weight, and food and water intake of WT and Trpc3 −/− mice at 2 and 18 months of age. On the right of (c), representative photographs of WT and Trpc3 −/− mice. f) Sucrose preference of WT and Trpc3 −/− mice at 2 and 18 months of age. g) HbA1c in WT and Trpc3 −/− mice at 2 and 18 months of age. h) Raw scatter plots of blood glucose after insulin administration to WT and Trpc3 −/− mice. i,j) Curve representation of blood glucose (w/o normalization) after insulin administration to WT and Trpc3 −/− mice. On the right: curve representation of raw blood glucose data. k) Western blots and quantifications of protein kinase B (pan‐AKT) and phospho‐pan‐AKT (p‐AKT T308) in WT and Trpc3 −/− mouse tissues ( n = 3). n = 25–50 mice in each condition. * p < 0.05, ** p < 0.01, and **** p < 0.0001 versus WT. Repeated measures two‐way ANOVA were used followed by Sidak's tests in (b)–(j). Unpaired two‐tailed t ‐tests were used in (k).

Article Snippet: HbA1c plasma concentration was determined by the ELISA technique according to the manufacturer protocol (Mouse glycated hemoglobin A1c Elisa kit, Cusabio, China).

Techniques: Staining, Western Blot, Two Tailed Test

Journal: Advanced Science

Article Title: TRPC3 Regulates Islet Beta‐Cell Insulin Secretion

doi: 10.1002/advs.202204846

Figure Lengend Snippet: Pharmacologic modulation of TRPC3 by a small‐molecule activator ameliorates experimental type 2 diabetes. a–d) Body weight, fasting blood glucose, HbA1c, and plasma insulin in wild‐type (WT) control and diabetic mice (T2D) w/o GSK1702934A. e,f) Raw scatter plots and curve representation of the average of plasma glucose concentrations during IpGTT in the different animal groups. On the right in (e): curve representation of raw plasma glucose concentration data. g) Area under curve (AUC) of the average plasma glucose concentrations in (e) and (f). h) Western blots and quantifications of protein kinase B (pan‐AKT) and phospho‐pan‐AKT (p‐AKT T308) in WT control and T2D mice w/o GSK1702934A ( n = 3). i) Representative organ sections stained with H&E from WT control and T2D mice w/o GSK1702934A. j) Histological analysis of mice tissues and liver weights in the different animal groups. n = 5 mice for each group. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 versus WT T2D. Brown–Forsythe and Welch ANOVA were used followed by Dunnett's T3 tests in (a)–(c). One‐way ANOVA were used followed by Tukey's tests in (d), (g), and (h). Repeated measures two‐way ANOVA were used followed by Sidak's tests in (e) and (f). Kruskal–Wallis one‐way ANOVA on ranks or ordinary one‐way ANOVA were used followed by either Dunn's or Tukey's multiple comparisons tests in (j).

Article Snippet: HbA1c plasma concentration was determined by the ELISA technique according to the manufacturer protocol (Mouse glycated hemoglobin A1c Elisa kit, Cusabio, China).

Techniques: Clinical Proteomics, Control, Concentration Assay, Western Blot, Staining

Journal: Nature Communications

Article Title: Long-term statins administration exacerbates diabetic nephropathy via ectopic fat deposition in diabetic mice

doi: 10.1038/s41467-023-35944-z

Figure Lengend Snippet: All mice were ~50 weeks old. a Schematic diagram showing the procedure of long-term administration of statins for db/db mice. b Body weight. For 12 weeks, n = 6 biologically independent mice in Db/m group, n = 13 in Db group, n = 10 in Db + Ato5 group, n = 16 in Db + Ato10 group, n = 9 in Db + Rosu20 group. But over time, some of the mice gradually lost weight and died. By 43W, n = 6 biologically independent mice in Db/m group, n = 6 in Db group, n = 5 in Db + Ato5 group, n = 3 in Db + Ato10 group, n = 3 in Db + Rosu20 group. For specific values see as a “Source Data file”. c Kaplan–Meier survival curves of db/db mice after long-term administration of statin. Log-rank (Mantel–Cox) test was used for the analysis of statistical significance. Exact p value = 4 × 10 −7 . d Measurements of fasting blood glucose. n = 4 per time point. Significance ** p < 0.01 compared with Db + Ato5 group, ### p < 0.001 compared with Db + Ato10 group; e GHbA1c levels. n = 6 in each group. f ITT and AUC for db / db mice at 40 weeks. n = 4 per time point, and n = 5 per time point i n Db + Ato5 group. g Immunohistochemical images of the RAGE in kidney sections. RAGE positive staining was mainly localized in the plasma membrane domains, and arrows represent RAGE expression in the cytoplasm of renal tubular epithelial cells . Original magnification ×400. Scale bar: 50 µm. All image part of the kidney was cortex. Renal structures indicated as proximal tubule (PT). h Quantitative analysis of expression of RAGE. n = 6 in each group. Data are expressed as means ± SEM ( b , d , e , f , h ). Significance tests were two-tailed, one-way ANOVA followed by Tukey’s test was performed ( b , d – f , h ). Source data are provided as a Source Data file.

Article Snippet: The serum fasting insulin levels and Glycated Hemoglobin A1c (GHbA1c) levels were examined using an ELISA kit (E08141m, Cusabio, Wuhan, China).

Techniques: Immunohistochemical staining, Staining, Clinical Proteomics, Membrane, Expressing, Two Tailed Test

Journal: Science Advances

Article Title: Label-free quantitation of glycated hemoglobin in single red blood cells by transient absorption microscopy and phasor analysis

doi: 10.1126/sciadv.aav0561

Figure Lengend Snippet: ( A and B ) Protein structure of the glycation site of HbA1c, and normal Hb, respectively. The crystal structure of HbA1c (3B75) and Hb (1LFZ) are from Protein Data Bank. Cyan sphere, water molecule; red sphere, oxygen atom; green sphere, carbon atom; blue sphere, nitrogen atom. In (A), the polar force between glucose and heme and surrounding water is highlighted by blue dashed lines. Regions of interest (ROIs) are highlighted by red dashed circles. ( C and D ) Zoom-in view of the interaction between glucose and porphyrin ring from glycated Hb (C) and normal Hb (D).

Article Snippet: Transient absorption imaging of whole blood study was achieved by measuring the HbA1c fraction from type 2 diabetic human whole blood (Lee Biosolutions, Inc.) and healthy human whole blood (Boston Children’s Hospital Blood Donor Center).

Techniques:

Journal: Science Advances

Article Title: Label-free quantitation of glycated hemoglobin in single red blood cells by transient absorption microscopy and phasor analysis

doi: 10.1126/sciadv.aav0561

Figure Lengend Snippet: ( A and B ) Fluorescence spectra of Hb (0.025 mg/ml) (A) along with HbA1c (0.025 mg/ml) (B), respectively. Excitation wavelength, 447 nm; integration time, 1000 s; band-pass filter, 488 ± 10 nm; power on the sample, 150 μW. 20× air objective. ( C and D ) Time-resolved photoluminescence (PL) measurements of Hb (0.025 mg/ml) (C) and HbA1c (0.025 mg/ml) (D), respectively. a.u., arbitrary units. ( E and F ) Absorption spectra (normalized) of Hb (E) and HbA1c (F), respectively.

Article Snippet: Transient absorption imaging of whole blood study was achieved by measuring the HbA1c fraction from type 2 diabetic human whole blood (Lee Biosolutions, Inc.) and healthy human whole blood (Boston Children’s Hospital Blood Donor Center).

Techniques: Fluorescence

Journal: Science Advances

Article Title: Label-free quantitation of glycated hemoglobin in single red blood cells by transient absorption microscopy and phasor analysis

doi: 10.1126/sciadv.aav0561

Figure Lengend Snippet: ( A and B ) Time-resolved decay curves (normalized) of Hb (A) and HbA1c (B), respectively. int., intensity. ( C ) Merged time-resolved curves (normalized) of Hb and HbA1c. ( D and E ) Time-resolved decay curves (normalized) of oxyHb (D) and oxyHbA1c (E), respectively. ( F ) Merged time-resolved curves (normalized) of oxyHb and oxyHbA1c. ( G ) Proposed excited-state dynamic pathway of Hb when pumped at 520 nm and probed at 780 nm.

Article Snippet: Transient absorption imaging of whole blood study was achieved by measuring the HbA1c fraction from type 2 diabetic human whole blood (Lee Biosolutions, Inc.) and healthy human whole blood (Boston Children’s Hospital Blood Donor Center).

Techniques:

Journal: Science Advances

Article Title: Label-free quantitation of glycated hemoglobin in single red blood cells by transient absorption microscopy and phasor analysis

doi: 10.1126/sciadv.aav0561

Figure Lengend Snippet: ( A ) Time-resolved decay curves (normalized) of standard HbA1c solutions (human whole blood based) at different concentrations. ( B ) Zoom-in view of (A) from delay time of 1 to 4 ps. ( C ) Component s versus different ω from 0 to 2π THz for pure HbA1c and Hb. ( D ) Phasor plot of standard HbA1c solutions of different concentrations when ω = 0.8π THz. ( E ) Calibration curve of standard HbA1c solution at different concentrations (component s versus HbA1c%).

Article Snippet: Transient absorption imaging of whole blood study was achieved by measuring the HbA1c fraction from type 2 diabetic human whole blood (Lee Biosolutions, Inc.) and healthy human whole blood (Boston Children’s Hospital Blood Donor Center).

Techniques:

Journal: Science Advances

Article Title: Label-free quantitation of glycated hemoglobin in single red blood cells by transient absorption microscopy and phasor analysis

doi: 10.1126/sciadv.aav0561

Figure Lengend Snippet: ( A ) Pseudocolor transient absorption images (delay time, 0 ps) of single RBCs with ROIs are highlighted by blue dashed circles. Scale bar, 10 μm. Pump: 520 nm, 2 mW on the sample; probe: 780 nm, 10 mW on the sample. int., intensity. ( B and C ) Time-resolved decay curves (normalized) of ROIs shown in (A). ( D to F ) HbA1c fraction (in single RBCs) distribution along with the fitted glucose concentration from three diabetic whole blood samples. ( G to I ) HbA1c fraction (in single RBCs) distribution along with the derived glucose concentration from three healthy whole blood samples. Curve fitted by .

Article Snippet: Transient absorption imaging of whole blood study was achieved by measuring the HbA1c fraction from type 2 diabetic human whole blood (Lee Biosolutions, Inc.) and healthy human whole blood (Boston Children’s Hospital Blood Donor Center).

Techniques: Concentration Assay, Derivative Assay